Effect of Cyclodextrins, Cholesterol, and Vitamin E Complexation on Cryopreserved Epididymal Ram Semen (2016): This study, published in Small Ruminant Research, investigates how the complexation of cyclodextrins with cholesterol and vitamin E influences the cryopreservation outcomes of epididymal ram semen. The research demonstrates that these compounds can improve membrane integrity and motility, essential factors for semen viability post-thawing.
Beneficial and Harmful Effects of Cyclodextrin-Vitamin E Complex on Cryopreserved Ram Sperm (2018): Published in Animal Reproduction Science, this research further evaluates the dual nature of cyclodextrin-vitamin E complex on sperm quality during cryopreservation. While the complex enhances antioxidant properties, it also highlights potential adverse effects at higher concentrations, thus offering insight into optimizing sperm preservation techniques.
Complementary Effect of Cholesterol and Vitamin E Preloaded in Cyclodextrins on Frozen Bovine Semen (2018): In CryoLetters, Benhenia and colleagues analyze how loading cholesterol and vitamin E in cyclodextrins improves frozen bovine semen’s motility parameters and membrane integrity while reducing lipid peroxidation, advancing the field of bovine reproduction preservation.
Use of Rosmarinus officinalis Essential Oil Preloaded in β-Cyclodextrin on Ram Spermatozoa (2019): This work investigates the effect of rosemary essential oil complexed with β-cyclodextrin on sperm quality. The study highlights the benefits of using natural antioxidants to preserve sperm motility and membrane integrity, contributing to non-synthetic preservation methods.
Research on Local Algerian Livestock: Benhenia has also contributed to characterizing Algerian livestock, including studies on the morphogenetic traits of local goats (Livestock Research for Rural Development, 2021) and Arab-Barb horses (Revue Méd. Vét, 2018). These studies play a crucial role in understanding and preserving regional genetic resources.
Innovative Cryopreservation Techniques: His work extends to developing novel cryopreservation methods, such as the optimization of ram sperm cryopreservation through encapsulating antioxidants in cyclodextrins (École Nationale Supérieure Vétérinaire, 2021).
Other Contributions: Dr. Benhenia has investigated the impacts of partially substituting barley with olive-waste cake on ram reproduction performance (Acta Veterinaria Eurasia, 2022) and explored ultrasonography for gestational age determination in Arab-Barb mares.